Regulating Hippocampal Plasticity: From Dynamics of Single Synapses to Alzheimer's Disease

A persistent challenge in unraveling mechanisms that regulate memory function is how to bridge the gap between inter-molecular dynamics of single proteins, activity of individual synapses and emerging properties of neuronal circuits. To achieve this goal, we developed an integrated system that enables simultaneous real-time visualization of structural reorganization in spatially-restricted signaling complexes and functional modifications of single synapses in brain circuits. We utilize FRET spectroscopy, high-resolution optical imaging, electrophysiology, molecular biology and biochemistry to explore experience-dependent mechanisms regulating the number and plasticity of hippocampal synapses under physiological and pathological conditions. Read More »